Ablation of arginyl‐tRNA ‐protein transferase in oligodendrocytes impairs central nervous system myelination

GALIANO, MAURICIO RAUL

GLIA

WILEY-LISS, DIV JOHN WILEY & SONS INC

Año: 2021 vol. 70 p. 303 - 303

0894-1491

ddition of arginine (Arg) from tRNA can cause major alterations of structure and function of protein substrates. This post-translational modification, termed protein arginylation, is mediated by the enzyme arginyl-tRNA-protein transferase 1 (Ate1). Arginylation plays essential roles in a variety of cellular processes, including cell migration, apoptosis, and cytoskeletal organization. Ate1 is associated with neuronal functions such as neurogenesis and neurite growth. However, the role of Ate1 in glial development, including oligodendrocyte (OL) differentiation and myelination processes in the central nervous system, is poorly understood. The present study revealed a peak in Ate1 protein expression during myelination process in primary cultured OLs. Post-transcriptional downregulation of Ate1 reduced the number of OL processes, and branching complexity, in vitro. We conditionally ablated Ate1 from OLs in mice using 2,3-cyclic nucleotide 3-phosphodiesterase-Cre promoter(?Ate1-KO? mice),